Coding

Part:BBa_M36306:Design

Designed by: Raman Nelakanti, Dennis Te, Darren Jindal   Group: Stanford BIOE44 - S11   (2011-12-08)

FseI restriction endonuclease


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 436
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 499
    Illegal PstI site found at 436
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 335
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 436
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 436
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization for use with E. coli


Source

Frankia species Eul1b (NRRL 18528)

References

Nelson,J.M., Miceli,S.M., Lechevalier,M.P. and Roberts,R.J. 1990. FseI, a new type II restriction endonuclease that recognizes the octanucleotide sequence 5′ GGCCGGCC 3′. Nucleic Acids Res., 18, 2061–2064. ([http://nar.oxfordjournals.org/content/18/8/2061.full.pdf])